STAR --runThreadN [threads] \ --genomeDir /path/to/index/ \ --readFilesIn read1.fastq read2.fastq \ --outFileNamePrefix SampleName_ Use code with caution. 4. Interpretation of the Report
To download and use the (Spliced Transcripts Alignment to a Reference) binaries to generate a report, follow the steps below. This report is commonly used in bioinformatics to align RNA-seq reads to a reference genome. 1. Download STAR Binaries Download STAR bin
Use the following command to download the static binary, which is the easiest to use as it has no external library dependencies: wget https://github.com chmod +x STAR Use code with caution. This report is commonly used in bioinformatics to
Download the latest release from and uncompress it. # Get latest STAR source from releases wget https://github.com/alexdobin/STAR/ STAR manual 2.7.0a Download the latest release from and uncompress it
High percentages (usually >70%) indicate high-quality data and successful alignment.
If you use Anaconda or Miniconda, you can install it through the Bioconda channel : conda install -c bioconda star Use code with caution. 2. Generate a Genome Index
The most direct way to obtain STAR is via its official GitHub repository.