A C4 ZipTip is a standard pipette tip containing a microscopic bed of resin fixed at its distal end. Unlike C18 resins, which are used for smaller peptides, the C4 resin is optimized for larger, more hydrophobic molecules like proteins and large polypeptides. Primary Uses in Research Researchers use these tools for two main reasons:
The C4 ZipTip is most commonly used in the workflow for (Matrix-Assisted Laser Desorption/Ionization Time-of-Flight). c4.zip
: Enrichment of dilute samples to ensure they are within the detection range of highly sensitive instruments. Role in Mass Spectrometry A C4 ZipTip is a standard pipette tip
: Typically optimized for very small volumes (microliters). : Enrichment of dilute samples to ensure they
: The protein sample is aspirated through the tip, binding to the C4 resin.
: The purified protein is eluted directly onto a target plate using an organic solvent like acetonitrile , where it is mixed with a matrix for analysis. Key Specifications Resin Type : Butyl (C4) silica.